CO2 and Orchids

 

Several months ago I began experimenting  with CO2. After dispelling several myths it has proven to be the easiest and most effective way to greatly improve the growth of  orchids. Flasks, compots and more mature plants respond equally well to these methods. I completed another chamber for flasks (12/19/01), I'm using a fish tank heater that is placed in a 1 gallon glass jar and placed inside the chambers for heat.

 I have much info on the subject and am placing links here as time permits. I would be interested in any constructive advice, comment or questions on the topic.

Pictures and instructions for my method of CO2 production by fermentation.


 

Myth #1....The growth curve graph in the article  indicates that levels of CO2 above 2000 ppm will become detrimental to plant growth.

Myth#2 ...In order to have a favorable affect growth, all other growth factors must be first maximized (temp, fertilizer and light). Then only by raising light levels will additional CO2 be utilized.

Carbon Dioxide Enrichment Methods While this article indicates levels above 2000 ppm are detrimental to plant growth it doesn't seem to apply to orchids. It has excellent info on the different types of CO2 enrichment methods which are:

Possible Effects on Red Phalaenopsis Hybrids by producing and storing starches in the mesophyll layer of the leaves. Red pigment(anthocyanins) are in solution in the cell vacuole and  need a good supply of starch/sugars while forming.

I just completed a 3' by 7' free standing CO2 chamber with shading out doors. It has been my recent observation that Phalaenopsis respond to ultra high levels of CO2 by growing much faster than those grown under ambient levels. Leaf tissue thickens with starch and sugar reserves, also aerial roots form and others branch. I have 4 of the 1 gallon glass containers with 1# of sugar plus 1 tea. of Active dry yeast fermenting in the labroom also. Plants in flask are growing much more rapidly and lush.

It has been reported that levels of CO2 above 2000ppm would inhibit growth-that's just not so. It is also said that high light and optimal nutrients are required before the increased CO2 will have a positive affect. That's also not my observation.  All plants in flask are growing much better regardless of light levels. My big concern was having that much live yeast in the Lab. Instead of a water lock I put a rubber stopper with a plastic tube going into a smaller container of 1 Tbls RD-20 and bubbled the CO2 through it. I had 9 flasks plugged into the flask manifold for 2 months and one became infected.

 

Journal Reviews from Center for the study of Carbon Dioxide and Global Change Good abstracts and insights that should be applicable to Orchids

     
Effects of Elevated CO2 on Photosynthesis in two CAM Plants Response of an Orchid to Super-Elevated CO2 Elevated CO2 Protects Photosynthesis at High Temperatures
Elevated CO2 And High Air Temperature: How Do They Affect Plant Pollen Germination? Environmentally-Stressed Plants Respond to Elevated CO2 Interactions Between Nitrogen, Water and Elevated CO2 for Six Perennial Plants
Elevated CO2 Doubly Stimulates Growth in Grasslands at Low Soil Moisture High Levels of CO2 and its affects on humans Interactive Effects of Water Stress and CO2 on Alfalf
Center Experiments

Experiment #1: Pothos in six 10-gallon aquariums

Experiment #2: Pothos in three double-3-liter bottles

Center Experiments

Experiment #3: Peas in 3-liter bottles in double-10-gallon aquariums

Experiment #4: Radishes in 3-liter bottles in double-10-gallon aquariums

 
 

 

Photosynthesis

Here are several e-mails collected by Bob Hamilton and located on Madison Heights Orchids website. This is very pertinate to orchids grown under high levels of CO2

 

 

A few facts and figures on Carbon Dioxide
ambient air has approximently 370ppm of CO2 or about .37 ml per liter CO2 = 44g/mole The volume of my lab is 1,215 ft3 or 33,980 liters
1 mole of CO2 is  about 22.4 liters in volume at STP(standard temperature and pressure) 1 lb. of granulated sugar is ~approximently 500ml At 370ppm there is 12.57liters(.444 ft3) of CO2  in the lab air
  1 lb. of sugar fermented will produce 1/2 pound of CO2 or 4.2ft3  
360ml/1 hour of CO2  produced by each of 4 generators(yeast) or 1.44 liters/hour Measurement Converter Here is a link to a small but very useful program that will help with converting volume, moles, density, light, and 16 other types of measurements

 

 
Growth promotion of coffee (Coffea arabusta)plantlets in vitro by use of fibrous supports containing no sugarand vessels with high number of air exchanges  http://www.nisshin.co.jp/seihin/rd/flo_ref1-1.html
Growth promotion of in vitro woody plants under photoautotrophic conditions http://www.nisshin.co.jp/seihin/rd/flo_ref_tsukuba.html
Clippings Galore/Orchids CO2 http://www.greeningearthsociety.org/Articles/2001/mowing.htm
Here is an article/e-mail concerning the use of ulta high levels of Carbon Dioxide. It was posted by Ed Herman, Editor and Publisher Agricell Report.µ
Link to a good measurement convertor http://www.joshmadison.com/software/convert/download.asp

 

 

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Above....Current new flask chamber...built 12/17/01

The Mineral Oil and Lithium Grease above or Vaseline is used to stop any mites that may be wondering around. I finger paint about 1 ft. square gridlines with it. This is done for all flask shelves. Why?....Several years ago I put some flasks outside for several months and got mites in a few of them. They spread to a few adjacent flask. I have been mite free since implimenting this. 

 

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Flask chamber.... 12/16/01Open at top
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This above is now the largest growth chamber. The stand-up flasks in the back contain a liquid media with spagnum moss and perlite for plant support instead of agar. MS and V&W media with sugar and also without sugar are being tested now. Hopefully the plants growing phototropically will need little if any time to acclimate when removed from flask. The absence of sugar in the media will also hinder flask contamination.Growth promotion of coffee (Coffea arabusta)plantlets in vitro

     
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Inside the 1/2 gallon glass container I put 1lb. of sugar, 1 tea. Active Dry Yeast and fill to that level with warm water.
     
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I had originaly cut a series of 2" holes into this 4" sqaure PVC fence post to make another CO2 flask manifold. Thought it might be interesting to percolate ultra high levels of CO2 thru the many holes in the bottoms of these plastic cups. The ends are closed and CO2 flows into the center of the post.